Animals

All the fish experiments were performed under the ethical guidelines of Cincinnati Children’s Hospital Medical Center; animal protocols were reviewed and approved by Cincinnati Children’s Hospital Medical Center Animal Care and Use Committee (Protocol # 2020-0031). Transgenic Tg(ubi:ERK-KTR(NLS3)-mTagBFP)^ci304 (generated in this study, Extended Data Fig. 2a-​-c),c), Tg(her7:her7-Venus)^ci303 (generated in this study, Extended Data Fig. 2d-​-l),l), Tg(hsp70l:HA-her1;ef1α:EGFP)¹⁴, and mutant Df(Chr05:her1,her7)^{b567/+ 25}, her1^ci301her7^{hu2526 30}, as well as AB wild-type adult fish were used in experiments. Fish were bred and maintained at 28.5°C on a 14/10-hour light/dark cycle. Non-transgenic littermate controls were used in heatshock experiments. Embryos were manually dechorionated in fish system water using needle tips for all experiments.