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Migration of SSCs stimulated by 25 nM CXCL12(1–68) and (3–68) was assessed using a scratch wound healing assay.71,72 Cells were grown in 24-well plates until confluence. A scratch was created in each well using a 300 μL pipette tip. Images were taken on an Olympus IX73 microscope (Olympus Scientific Solutions Americas Corp., Waltham, MA, USA) 24 h after treatment. The scratch wound healing % was quantified by measuring the average width of the scratch at time 0 and 24 h using open-source image analysis software ImageJ.
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