2.3. Cells Culture

All stages of the experiment were performed under aseptic, standardized culture conditions (37°C, 5% CO₂). Primary cultures were plated on T25 culture flasks in culture media consisting of Dulbecco's Modified Eagle's Medium (DMEM) with nutrient F-12 Ham, 10% of Fetal Bovine Serum (FBS), and 1% solution of P/S/A. The medium was changed every two days, while passage of cells was performed after reaching 80–90% confluence. Prior to the experiment, cells were passed three times using trypsin solution (TrypLE™; Life Technologies, Carlsbad, USA) in accordance with manufacturer's protocol.