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Based on our previously established method (Bu et al., 2016), western blotting was employed to detect the protein levels of StAR in cultured GCs. The cell lysates were resolved by electrophoresis in a 15% SDS-PAGE and the bands from western blotting were quantitated by densitometric analyses (Image J software, NIH). The relative StAR levels were calculated as the ratio to that of β-actin and then expressed as the percentage of the control group (without CORT treatment).
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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