2.3. Minimum leaf conductance (g min)

LS Leonie C. Schönbeck
PS Philipp Schuler
ML Marco M. Lehmann
EM Eugénie Mas
LM Laura Mekarni
AP Alexandria L. Pivovaroff
PT Pascal Turberg
CG Charlotte Grossiord
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Minimum leaf conductance (Kerstiens, 1996) was measured as described in Pearcy and Zimmermann (2000). One leaf per individual was cut before dawn when stomata were assumed to be still closed. The cut petiole was immediately sealed with melted candle wax, and the leaf area was scanned using a flatbed scanner, followed by analysis using Pixstat (Schleppi, 2021). The leaves were stuck to a lab tape run between two lab stands, standing in a small dark climate chamber with stable T (26°C) and humidity (60%) and the ventilation on. Every 15–20 min, the leaves were taken from the climate chamber and weighed in a dark room using a fine‐precision scale (Mettler‐Toledo). This procedure was repeated eight times. g min (mmol m−2 s−1) was calculated as cuticular transpiration per mole fraction VPD, assuming the leaf internal air to be fully saturated (Pearcy & Zimmermann, 2000).

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