3.2. Preparation of the N-Hexane Extracts of Various Organs

The dried samples of the barks, leaves, seeds, and fruits of T. indica (100 g each) were powdered and extracted by cold maceration method with n-hexane (500 mL × 3) separately, followed by filtration for 3 days. The filtrate of each plant material was completely evaporated in vacuo at 45 °C until dry to obtain the dried residue of the n-hexane extract (420 mg, 610 mg, 207 mg, and 312 mg) for barks, leaves, seeds, and fruits, respectively. The dried residue of each plant part was named as follows; T. indica bark (TIB), T. indica leaves (TIL), T. indica seeds (TIS), and T. indica fruits (TIF). All extracts were stored in a tight container at 4 °C in the refrigerator for further analysis [65].