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2.7. Detection of Glomerular PLA2R, Thrombospondin Type 1 Domain-Containing 7A(THSD7A), and Neural Epidermal Growth Factor–Like 1(NELL-1) Expression and Circulating Anti-PLA2R Antibody
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Immunohistochemistry staining for PLA2R, THSD7A and NELL-1 was performed as previously described [9,13,14]. The rabbit anti-human PLA2R polyclonal antibody (1:800; Sigma-Aldrich), the rabbit anti-human THSD7A polyclonal antibody (1:1500; Sigma Chemical Company and the rabbit polyclonal anti–NELL-1 antibody (1:800; Sigma Chemical Company) were the primary antibodies. The serum anti-PLA2R antibody levels were detected by ELISA (EUROIMMUN, Lübeck, Germany). The results were considered positive at ≥ 20 relative units (RU)/mL.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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