Cells (GIST-T1: 5.0 × 103; HMC1.2: 1 × 104) were seeded in 96-well plates. Each compound was treated in each well at 10 dose points of 3-fold serial dilution. After treatment with each compound for 72 h, CellTiter-Glo solution (Promega, # G7572) was added to each well. Anti-proliferation activity was measured the luminescence using a 96-well plate reader (EnVision 2013).
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