2.9. GO Enrichment and KEGG Pathway Enrichment Analysis

DEGs performed Gene Ontology (GO) and KEGG functional enrichment analysis using the Goseq R software package and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis using the KOBAS online tool (http://kobas.cbi.pku.edu.cn/genelist/, accessed on 13 July 2022), respectively. A value of p < 0.05 represented a significant difference. Finally, we visualized the enrichment analysis results of GO (top 13 biological processes, 6 cellular components, and 5 molecular functions) and KEGG (top 25), based on the p-values. GO enrichment analysis circle plot is plotted by the online site ChiPlot (https://www.chiplot.online/, accessed on 13 July 2022). The outermost layer of the GO enrichment analysis circle plot represents the enriched GO ID. The second layer represents the number of background genes in the pathway, and the depth of the color represents the size of the p value. The third layer represents the Rich factor, with red representing the biological process, light green representing the cellular component, and earthier yellow representing the molecular function. The KEGG pathways plot was created using the R package ggplot2 (https://ggplot2.tidyverse.org, accessed on 14 July 2022).