2.2. Locomotor Activity

Five or six mice of each genotype from a separate group of mice were singly housed in individual cages outfitted for locomotor activity analysis via detection of infrared beam breaks. These mice were fed either RC or HF diet under LD 12 : 12 or LD 10 : 10 cycle for 10 days and were used exclusively for locomotor activity analysis (i.e., they were not included in the body weight measurements or other metabolic analyses). Beam breaks were recorded in 6 min bins using the Chronobiology Kit (Stanford Software Systems, Stanford, CA, USA) and were analyzed using the ClockLab software (Actimetrics, Wilmette, IL, USA).