Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
A cell-based ELISA assay kit (Abnova, Taoyuan, Taiwan, China) was used to measure the ERK1/2 phosphorylation level in Caco-2 cells. Caco-2 cells cultured in 96-well black side plates were used to perform ELISA according to manufacturer’s instructions. Fluorescence intensity was measured at 535/590 nm for ERK1/2 and 360/450 nm for total proteins using a microplate spectrophotometer to assess the normalized ERK1/2 phosphorylation level.
Copyright and License information: ©2022 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
