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Chrome azurol S (CAS) assay for siderophore detection
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The medium for 1 L CAS was prepared according to the method of Schwyn and Neilands (1987)51 without the addition of nutrients: 60.5 mg CAS, 72.9 mg hexadecyltrimethyl ammonium bromide (HDTMA-Br), 30.24 g piperazine-1,4‐bis(2‐ethanesulfonic acid) (PIPES) and 10 mL of 1 mM FeCl3·6H2O in 10 mM HCl. Agarose (0.9% w/v) was used as gelling agent. The CAS agar solution was overlaid onto the spots of Streptomyces sp. MBT42. Following incubation overnight at 30 °C (±18 h) the plates were examined visually for halos.
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