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In the two controlled hemorrhage model development animals, arterial blood samples from the animal before hemorrhage, samples from the post-suction blood bag, and arterial blood samples from the animal post-transfusion were obtained. In the two controlled hemorrhage experimental animals, samples from the baseline arterial blood, post-suction blood bag, immediately post-retransfusion, and every 30-min during resuscitation were obtained. ABG, Chem8+, and activated clotting time (ACT) were analyzed in all animals.
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