2.2. Synthesis of selenium nanoparticles (SeNPs)

Five milliliters of Adiantum capillus extract was diluted with 45 mL of 12 mM of Sodium selenite (Na₂SeO₃). The mix was stirred at 60 °C for three days until a yellow to ruby red color was seen. Then the product was washed with Deionized distilled water and centrifuge at 10,000 rpm for ten minutes (Pyrzynska and Sentkowska, 2021). Followed by a second washing of Phosphate buffer solution (pH 7.4) and ultra-sonication several times until the impurities were removed. At the last, a light red pellet was dried in oven for two hours. and stored at −20 °C until use (Cittrarasu et al., 2021).