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Fluorescence recovery after photobleaching (FRAP)
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A FRAP analysis was performed to study the formation and fluidity of the lipid bilayers. The bilayer was prepared as mentioned above using egg PC mixed with Texas Red dye-DOPC. Images were recorded prephotobleaching, after closing the iris aperture, and postphotobleaching at an exposure time of with a excitation LED lamp (Thorlabs, Newton, NJ, USA, DC4100 4-Wavelength LED Source) operated at its maximum constant current mode of 1,000 mA.
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