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Measurement of urinary corticosteroids
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The urinary samples for determination of urinary free cortisol (UFF), urinary free cortisone (UFE), urinary total cortisol (UTF), urinary total cortisone (UTE), and their metabolites 5alpha- or allo-tetrahydrocortisol (aTHF), 5beta-tetrahydrocortisol (THF), and tetrahydrocortisone (THE) were analyzed using a validated LC-MS/MS method. The ratio (aTHF+THF)/THE was used to indirectly assess 11beta-HSD1 inhibition in the liver.
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