RNA immunoprecipitation (RIP)‐qPCR

Cal27 and SCC4 cells were lysed with RIPA lysis buffer (Solarbio, Beijing, China). Binding of YTHDC1 or GATA3 to MEG3 was detected using anti‐YTHDC1 (#ab264375, Abcam), anti‐GATA3 (#ab199428, Abcam), and anti‐IgG (#ab133470, Abcam) and the Magna RIP Kit (Millipore, Billerica, MA, USA) according to the manufacturer's protocol. The m6A modification of MEG3 was detected using anti‐m6A (#ab208577, Abcam), anti‐IgG (#ab133470, Abcam), and Magna MeRIP™ m6A Kit (Millipore) according to the manufacturer's instructions. Relative enrichment of RNA was measured by qRT‐PCR. The sequences of primers were as follows: MEG3, forward, 5'‐CTTTTCTGGGGGAATGGGG‐3′; reverse, 5'‐AGAGGGGTGGGAAGGGACT‐3′.