2.4. Vancomycin Analysis

Before analysis, the researcher verified that simultaneous sampling collection was obtained within 4 hours and that blood was not hemolysed. When the blood was hemolysed or 4 hours between the collection and analysis was exceeded, the samples were discarded and not used for analysis. Blood from LiHep syringes was transferred into a tube for further analysis at the hospital's pharmacy laboratory. All blood samples were collected and centrifuged at 1811g for 6 min. Vancomycin was analyzed by the validated method using a particle-enhanced turbidimetric inhibition immunoassay (PETINIA) autoanalyzer, Abbott Architect C4000 (Chicago, IL, USA) and Abbott vancomycin assay (6E44-21, Chicago, IL, USA) were used. The TDM Abbott multiconstituent calibrator was used to calibrate the vancomycin assay (05P0401, Chicago, IL, USA).