Dynamic light scattering

Light scattering data were acquired at 298 K using a DynaPro Plate Reader III (Wyatt Technology, Santa Barbara, CA) in a 384-well plate format. Samples (60 μL total volume in 20 mM NaPO₄, 150 mM NaCl, pH 7.5) contained 2 μM Spike protein and increasing BOA concentrations (32 nM to 2 μM). For each sample ten sets of scattering data were collected and averaged in Dynamics 7.1.7. Data for each concentration were collected in triplicate. Hydrodynamic radii were extracted, and the data was analyzed in MatLab. The errors are given as the standard deviation of the mean for 3 replicates.