2.6. Enzyme-Linked Immunosorbent Assay (ELISA) for Cytokines

HL Hui-na Lu
ZF Zhou Fu
XC Xia Chen
MY Ming-ming Yang
YC Yun-fang Chen
LY Li-li Yang
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After thoroughly mixing, the obtained BALF was centrifuged at 1800 rpm, and the supernatant was used to detect the change in cytokine levels (IL-4, IL-13, IL-33, and TGF-β) using an ELISA kit (Neobioscience, China), according to the manufacturer's instructions.

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