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Measurement of IL-1β, IL-6 and TNF-α concentration in the RAW 264.7 macrophage cell line
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The RAW 264.7 macrophage cells (104cells in each well of 96-well plates) were pre-incubated with fluoxetine (5, 10, or 20 µg/mL) for one hour before LPS (1 μg/mL) administration for 24 h at 37 °C. The culture media were then collected and centrifuged at 800g to obtain the supernatants. The levels of IL-1β, IL-6, and TNF-α were assessed by commercial ELISA kits (R&D Systems, USA; Cat No., IL-1β: SMLB00C; IL-6: SM6000B; and TNF-α: SMTA00B). A microplate reader (BioRad, USA) was used to read absorbance at 450 nm.
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This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
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