Cell lysis using 1x RIPA buffer

This lysis protocol was followed prior to sample analysis with the Wes™ system. Ice cold 1x RIPA buffer was added to the cell pellets and the cells were resuspended in the buffer. Unless already performed, the cells in the buffer were transferred to separate 1.5 ml tubes. Lysis was performed for 15 min on ice. The lysates were centrifuged 5 min at 16,000 ×g and 4°C to remove cell debris, and the supernatants (lysates) were transferred to fresh 1.5 ml tubes. The lysates were stored at −80°C until analysis.