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Intracellular cytokine staining

TD Timothy Donnison
JM Joey McGregor
SC Senthil Chinnakannan
CH Claire Hutchings
RC Rob J. Center
PP Pantelis Poumbourios
PK Paul Klenerman
HD Heidi E. Drummer
EB Eleanor Barnes
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Intracellular cytokine staining (ICS) was performed as described.[ 24 ] Gating/analysis were performed in FlowJo (v10.7; TreeStar, Ashland, OR). Cytokine coexpression within T‐cell subsets was determined using Boolean gating in FlowJo and graphs produced in Pestle (v2.0), and SPICE (v6.0; NIAID, NIH).

Copyright and License information:  ©2022 The Authors. published by Wiley Periodicals LLC on behalf of American Association for the Study of Liver Diseases
This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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