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2.4. Assessment of oocyte viability
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Oocytes were stained for 2 min in 2.5 μg/mL 2′,7′‐dichlorodihydrofuorescein diacetate (FDA; Thermo Fisher Scientific, Massachusetts, USA) in TL‐HEPES‐PVA. After three washings in TL‐HEPES‐PVA, oocytes were examined under a fluorescence stereomicroscope (Nikon SMZ18; Nikon, Tokyo, Japan). Viable oocytes showed bright green fluorescence, and viability was calculated as the percentage of viable oocytes to the total number of oocytes assessed.
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