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Liver lipids extraction and quantification
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Liver lipids were extracted from 100 mg of tissue homogenized with a 20-fold excess of a chloroform–methanol (2:1 by volume) mixture, as described by Folch17. The homogenates were filtered using filter paper, then lipid extracts were dried, and the pellet was dissolved in isopropanol solution. Liver concentrations of CHOL and TG were determined using commercial kits (Bioclin Quimica Basica Ltda, Belo Horizonte, MG, Brazil; cat No. #K083 and #K117, respectively), and were normalized per milligram of liver weight.
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