4.8. Determination of COX Activity

AV Ajjampura C. Vinayaka
NS Nurit Shalev
SA Seegehalli M. Anil
ST Sudeep Tiwari
NK Navin Kumar
EB Eduard Belausov
KM Karthik Ananth Mani
GM Guy Mechrez
HK Hinanit Koltai
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Inhibition of COX activity was determined using Cyclooxygenase 1 (COX1) Inhibitor Screening Assay Kit (ab204698, Abcam, Cambridge, UK) and Cyclooxygenase 2 (COX2) Inhibitor Screening Assay Kit (K547-100, BioVisionInc., Milpitas, CA, USA). The fluorometric detection method was applied in 10 µL COX assay buffer, according to manufacturer instructions.

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