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2.4.2. Determination of the Total Flavonoid Content

BT Barbora Tunklová
LJ Lukáš Jeníček
JM Jan Malaťák
MN Michal Neškudla
JV Jan Velebil
FH František Hnilička
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Total flavonoid content (TFC) was determined by the colorimetric method with aluminum chloride (Lachner, Neratovice, Czech Republic) according to the methodology previously described by Chia-Chi et al. [46] with slight modifications, using methanolic extracts. Absorbance samples were measured at a wavelength of 415 nm. The total flavonoid content was calculated from a calibration curve, and the result was expressed as quercetin equivalents (QE mg g−1 DW).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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