2.1. Materials

Crizotinib was kindly provided by Pfizer. Brigatinib (AP26113; Cat. #S8229) and lorlatinib (PF-6463922; Cat. #S7536) were purchased from Selleckchem (Houston, TX, USA). Silibinin (Cat. #S0417) was purchased from Sigma-Aldrich (Madrid, Spain). All reagents were dissolved in sterile dimethylsulfoxide (DMSO) to prepare 10 mmol/L stock solutions, which were stored in aliquots at −20 °C until use. Working concentrations were diluted in culture medium prior to each experiment.

Antibodies against E-cadherin (#3195), SMAD2/3 (#3102) and phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (#9510) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against GADPH (#60004-1-Ig) and β-actin (#66009-1-Ig) were purchased from Proteintech Group, Inc (Rosemont, IL, USA). Antibodies against vimentin (#V6630) and SNAIL (#MA5-14801) were purchased from Sigma-Aldrich and ThermoFisher Scientific Inc. (Waltham, MA, USA), respectively.

The Applied Biosystems^TM TaqMan^TM Array Human TGFβ Pathway 96-well Plate (Cat. #4414097) was purchased from Applied Biosystems (Foster City, CA, USA). RayBio^® C-Series Human TGFβ Array C2 (Cat. #AAH-TGFB-2-2) was purchased from RayBiotech, Inc. (Norcross, GA, USA).