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2.3. Detection of hUCMSC Surface Markers
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After three passages, hUCMSCs were examined for surface marker expression. Cells were trypsinized and washed with and resuspend in PBS to a density of 1 × 106 cells/mL. A volume of 100 μL of this cell suspension was added to 1.5 mL Eppendorf tubes. Tube 1 was used as a negative control (with PBS), and the other tubes were incubated with isotype control-FITC, CD73-FITC, CD90-FITC, CD105-FITC, CD14-FITC, CD34-FITC, or CD45-FITC antibodies for 30 minutes. The cells were then analyzed using flow cytometry.
Copyright and License information: ©2016 Yajing Huang et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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