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2.8. Bio-Layer Interferometry (BLI)

CH Craig A. Hokanson
EZ Emanuela Zacco
GC Guido Cappuccilli
TO Tatjana Odineca
RC Roberto Crea
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The Pronectin lead candidates were analyzed by BLI on the Forte Biosystem Octet QR. The AXL-Fc target proteins were loaded onto Protein A sensors at 25 µg/mL. Pronectins were added at 10 µg/mL. The Kon, Koff and KD for the Pronectins were calculated by the software.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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