2.10. Parallel Reaction Monitoring (PRM) Analysis

The peptide digests were separated and analyzed using Easy nLC 1200 (Thermo Scientific, Waltham, MA, USA) and Q-Exactive HFX (Thermo Scientific, Waltham, MA, USA), respectively. The target protein data were imported into the Skyline 4.1 software (Sciex, Toronto, ON, Canada). The raw file was imported into the original data of data dependent acquisition (DDA) by MaxQuant software (Martinsried, Germany) to screen the matching peptides with high ionic strength and fewer heteropeaks. Database retrieval parameters were set as follows: missed cleavage was set to 0, and the reliability of the peptide was ≥0.95. Finally, the database search results were imported into Skyline software to compare the selected candidate peptides.