2.6.1. Simulated Gastrointestinal (GI) Digestion Using Peptide Cutter

In silico analysis was used to determine potential survival of identified ACE-I inhibitory peptides in the GI tract. The four identified peptide sequences were assessed for potential cleavage by GI tract enzymes as described previously [24,27,28] using the software program Expasy PeptideCutter http://ca.expasy.org/cgi-bin/peptidecutter/peptidecutter.pl-accessed on the 25 July 2022. Simulated cleavage of the peptides with pepsin (pH > 1.3 and pH 2.0) (EC 3.4.23.1) and trypsin (EC 3.4.21.4) was carried out. Resulting peptide fragments were subsequently assessed for potential bioactivities and novelty in BIOPEP-UWM.