2.7.3. Acclimation Potential

The acclimation potential of NP and DR, the acclimation ratio (AR), was calculated by dividing rates from the treatment samples at growth temperature (5 °C higher than controls) by rates from the control samples at growth temperature (Rate_{treatment at T+5°C}/Rate_{control at T}) at each temperature step. According to Larigauderie and Koerner [22], an AR of 1.0 indicates acclimation, i.e., the DR or NP at the identical test temperature of plants grown at the higher temperature is the same as those grown at the lower temperature. For DR, values greater than 1.0 indicate partial or no acclimation. In situations where acclimation is occurring, the DR temperature response curve of the treatment samples would be lower than the control rates at any selected temperature. If partial acclimation has occurred, then AR will be less than control group Q₁₀ for the same temperature range [22]. Q₁₀ values for the DR response curves were calculated using an interactive table at https://www.physiologyweb.com/calculators/q10_calculator.html (accessed on 3 August 2022). For NP, acclimation is indicated by identical or greater rates of the treatment samples in comparison to the controls, with potential shifts in the temperature response curve [74]. Therefore, values greater than 1.0 indicate acclimation through increased rates (potentially in combination with shifted response curves) while values less than 1.0 indicate a lack of acclimation.