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Preparation of BM derived macrophages

TO Takanori Ochi
YF Yongjia Feng
SK Sho Kitamoto
HN Hiroko Nagao-Kitamoto
PK Peter Kuffa
KA Koji Atarashi
KH Kenya Honda
DT Daniel H. Teitelbaum
NK Nobuhiko Kamada
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BM cells were isolated from mouse femurs and tibias. After isolation, BM mononuclear cells were cultured for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM) containing 10% FBS, 1% penicillin/streptomycin, 2-mercaptoethanol (25 μM), sodium pyruvate (1 mM), MEM non essential amino acids (NEAA) (all from Life technologies) and 30% L-cell conditioned medium. After differentiation, cells were collected and washed two times with PBS59.

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