2.3. MTT assay

Hep3B, SUN387, and MHCC97 cells were seeded in 96-well plates at a density of 500–4000 cells/well and allowed to attach overnight before drug administration. After palbociclib, cisplatin, imatinib, and paclitaxel were applied to cells, MTT assay (3-(4, 5-dimethyl-1, 3-thiazole-2)-2, 5-dibenzo-2-hydrotetrazole-3-ammonium bromide; Aladdin, #J21061611) was hired to assess cell viability. MTT solution of 5 mg/mL was added to the medium and then incubated at 37 °C for 4 h. Absorbance was measured at OD 490 nm. The growth inhibition rate of cells in each well was calculated as (OD value control -OD value drug)/OD value control × 100%.