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AML cells were seeded in 96-well plates at 10,000 cells per well, and incubated with different concentrations of GSK269962A. After 72 h, 10 μl of CCK-8 solution was added to each well and incubated at 37°C for 2 h. The OD value at 450 nm was measured with a microplate reader. Background reading of the media was subtracted from each well for result standardization.
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