Antibodies

The following primary antibodies were used for western blot (WB), immunofluorescence (IF) and proximity ligation assays (PLA): mouse anti-β-actin (WB, 1:5000; Sigma, A5441), rabbit anti-HSP60 (WB, 1:1000; IF, 1:1000; Abcam, ab53109), goat anti-HSP60 (IF, 1/500; MyBioSource, MBS423631), mouse anti-ATP5F1B (WB, 1:1000, IF, 1:500; Abcam, ab14730), mouse antimitofusin 2 (WB, 1:1000; Abcam, ab56889), mouse anti-LC3B (WB, 1:2000; Novus Biologicals, NB100-2220), rabbit anti-RAB10 (WB, 1:1000; Cell Signaling Technology, 8127S), rabbit antiphospho-RAB10^T73 (WB, 1:500; Abcam, ab230261), rabbit anti-PINK1 (WB, 1:1000; Novus Biologicals, BC100-494), rabbit anti-optineurin (WB, 1:1000; IF, 1:200; Abcam, ab23666), mouse anti-optineurin (IF and PLA, 1:50; Santa Cruz, sc-166576), rabbit anti-DJ-1 (WB, 1:1000; IF and PLA, 1/250; Abcam, ab18257), rabbit antityrosine hydroxylase (TH) (WB, 1:500; IF, 1:500; Sigma, AB152), mouse anti-TH (IF, 1:500; Sigma, MAB318), sheep anti-TH (IF, 1:500; Thermo Fisher, PA1-4679), mouse anti-MAP2 (IF, 1:500; Sigma, M1406), mouse anti-PSD95 (IF, 1:500; Cell Signaling, 36233), rabbit antisynaptophysin (IF, 1:500; Thermo Fisher, MA5-14532), mouse anti-ubiquitin (WB, 1:50; Santa Cruz, sc-8017), rabbit antiphospho-ubiquitin^S65 (WB, 1:1000; IF, 1:250; Sigma, ABS1513-I), mouse anti-FLAG (WB, 1:1000; IF, 1:500; Sigma, F3165), rabbit anti-FLAG (WB, 1:1000; IF, 1:500 Sigma, F7425), goat anti-FLAG (IF, 1/500; Origene, TA150080), mouse anti-GTP cyclohydrolase 1 (PLA, 1:50; Abnova, H00002643-M01), mouse antiprotein disulphide isomerase (PLA, 1:500; Enzo Life Sciences, ADI-SPA-891), mouse anti-EEA1 (PLA, 1/500; BD Biosciences, 610456), rabbit anti-calnexin (PLA, 1/500; Enzo, ADI-SPA-860), mouse anti-GM130 (PLA, 1/200; BD Biosciences, 610822), rabbit anti-GOLGA5 (PLA, 1/500; Sigma, HPA000992), mouse anti-COPB1 (WB, 1:1000; Sigma, G6160), rabbit anti-TBK1 (WB, 1:1000; Cell Signaling, 3504S), rabbit antiphospho-TBK1^S172 (WB, 1:1000; Cell Signaling, 5483S), rabbit anti-NDP52 (WB, 1:1000; Cell Signaling, 60732S), rabbit anti-GFAP (ICC, 1:500; Agilent Dako, Z0334) and mouse anti-TOMM20 (ICC, 1:500; Santa Cruz, sc-17764). Peroxidase-linked secondary antibodies for WB were from Sigma (SAB3700934 and SAB3701095). Secondary antibodies for IF were donkey antimouse Alexa Fluor-488, −555 and −647 (Thermo Fisher, a21202, a21206, a31571), antirabbit Alexa Fluor-488, −555 and −647 (Thermo Fisher, a31570, a31572, a31573) and antisheep Alexa Fluor-488, −555 and −647 (Thermo Fisher, a11015, a21436, a21448).