Zymosan A–induced peritonitis model

To induce acute peritonitis, Tie2-KO or Ctrl mice were injected intraperitoneally with 10 μg of Zymosan A (from saccharomyces cerevisiae; Sigma) per mouse or PBS vehicle control. After 24 hours, mice were killed by cervical dislocation and a small incision was made to expose the peritoneum without damage. The peritoneal cavity was washed with 10 mL ice cold PBS and the lavage was carefully collected by avoiding any bleeding. The lavage volume was recorded and peritoneal lavage samples were subjected to flow cytometric analysis.