Elevated plus maze test

we used the elevated plus maze test to measure the rat’s movement and anxiety. It comprised 2 open arms (50×10 cm) and 2 enclosed arms (50×10×40 cm) that extend from a common central platform (10×10 cm). A wooden apparatus was elevated 50 cm above floor level. Testing was conducted between 9.00 am and 1.00 pm in a quiet room. The rats were placed in the center of the maze facing an open arm individually, and 5 min were allowed for free exploration. All sessions were filmed. The floor was cleaned after each test with distillate water. The animals were tested before the first ELF-MF exposure and after the last ELFMF exposure. Measurements were made from the time spent in the maze’s central, open and closed parts. In the absence of anxiety, the animal moves more and has a great deal of activity in the open arm. However, if the rat experiences anxiety and sleepiness, it will remain in the closed arm ( Rodgers & Dalvi, 1997).