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2.6. In vivo electrophysiology and induction of long-term potentiation (LTP)

SH Somoday Hazra
JH Joyeeta Dutta Hazra
RB Rani Amit Bar-On
YD Yanhong Duan
SE Shahaf Edut
XC Xiaohua Cao
GR Gal Richter-Levin
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The electrophysiology procedure was conducted as described before (Ardi et al., 2014; Tripathi et al., 2021) in the dentate gyrus –perforant pathway (DG-PP). Two TBS stimulation protocols were used - a regular TBS protocol and a strong TBS protocol. Regular protocol: a single set of 10 trains, each of 10 pulses, administered at 100 Hz with 200 ms inter-train interval. Strong protocol: three sets of 10 trains, each of 10 pulses, administrated at 100 Hz with 200 ms inter-train interval and 1 min inter-set interval. LTP was recorded for 60 min after TBS and measured as the difference in EPSP slope before and 60 min after TBS (For a detailed description, see Supplementary details).

Copyright and License information:  ©2022 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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