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PMSG Injection for Super-ovulated Mice

YM Yang Ma
YZ Yanyu Zhong
XC Xia Chen
HL Huijun Liu
YS Yichao Shi
XZ Xiuwen Zhang
HS Huiting Sun
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Female C57BL/6 mice (OC and OC + HBOT groups) were intraperitoneally injected with 5 IU PMSG (Prospec Tany, TechnoGene, Ltd.) to induce super-ovulation. Next, for in vitro fertilization (IVF) and embryo culture, the mice were sacrificed through cervical dislocation 48 h later after the PMSG injection. The IVF and embryo culture were performed as described elsewhere [18].

Copyright and License information:  ©2022-12-15 The Author(s), under exclusive licence to Society for Reproductive Investigation 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.

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