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Acquired images were processed using ImageJ/Fiji (Schneider et al., 2012). AID::GFP fluorescence was quantified as previously described (Martinez and Matus, 2020). DHB::2xmKate2 ratios were quantified as previously described (Adikes et al., 2020). AC number was determined by counting AC nuclei (LAG-2::P2A::H2B::mTurquoise2). AC invasion was defined as the complete loss of BM (LAM-2::mNeonGreen) under the AC. Plots were generated using Prism software. Figures, and the cartoons within, were created using a combination of Adobe Photoshop and Illustrator.
Copyright and License information: ©2022 2022. Published by The Company of Biologists Ltd
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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