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Cell culture

LZ Lina Zhang
SW Song Wu
JH Junjie Huang
YS Yanbin Shi
YY Yuesong Yin
XC Xu Cao
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Two osteosarcoma cell lines (U2OS and MNNG/HOS) were obtained from the Procell Life Science & Technology Co., Ltd. U2OS and MNNG/HOS were correspondingly cultured in McCoy’s 5A (Procell, China) and MEM (Procell, China) supplemented with 10% fetal bovine serum (FBS, Gibco, USA) and 1% penicillin-streptomycin solution (Biosharp, China) at 37°C with saturated humidity and 5% CO2. The average time of culture medium exchange was 24-48h. The cells were digested with trypsin-EDTA (Gibco, USA) and passaged when cell adhesion exceeded 80% confluency.

Copyright and License information:  ©2022 Zhang, Wu, Huang, Shi, Yin and Cao
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