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Normal phase LC/MS

YZ Yan-Bing Zhu
WG Weizhen Gao
YZ Yongbo Zhang
FJ Feng Jia
HZ Hai-Long Zhang
YL Ying-Zi Liu
XS Xue-Fang Sun
YY Yuhua Yin
DY Dong-Min Yin
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Polar lipids were analyzed using an Agilent 1260 HPLC system coupled with a triple quadrupole/ion trap mass spectrometer (5500Qtrap; SCIEX) as described previously39. Separation of individual lipid classes of polar lipids by normal phase (NP)-HPLC was carried out using a Phenomenex Luna 3μ-silica column (internal diameter 150 × 2.0 mm) with the following conditions: mobile phase A (chloroform: methanol: ammonium hydroxide, 89.5:10:0.5) and mobile phase B (chloroform: methanol: ammonium hydroxide: water, 55: 39: 0.5: 5.5). MRM transitions were set up for comparative analysis of various polar lipids. Individual lipid species were quantified by referencing to spiked internal standards. PC-14:0/14:0, PE-14:0/14:0, PS-14:0/14:0, PA-17:0/17:0, PG-14:0/14:0, LPA-17:0 and LPC-17:0 were obtained from Avanti Polar Lipids (Alabaster, AL) and LIPID MAPS.

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