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Mouse testes and small intestines were harvested. The entire testis was fixed with a special testis solution (Servicebio Cat. No.: G1121) for 24 h. The small intestine was cut into small sections of approximately 1 cm, which were then cleaned and fixed with paraformaldehyde (Servicebio Cat. No.: G1101) for 24 h. Hematoxylin and eosin (H&E) staining was performed after dehydration in graded ethanol solutions, paraffin embedding, and sectioning (2.5 μm). Three random fields of sections on each slide were observed under an Olympus upright microscope (×200).
Copyright and License information: ©2022 Li, Li, Lu, Yang, Zhao, Zhu, Yi, Fu, Luo, Tang, Zhang, Yang, Liu, Xu, Chen and Zhu.
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