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Dynamic light scattering (DLS)

AR Ayeshah A. Rosdah
BA Belinda M. Abbott
CL Christopher G. Langendorf
YD Yali Deng
JT Jia Q. Truong
HW Helen M. M. Waddell
NL Naomi X. Y. Ling
WS William J. Smiles
LD Lea M. D. Delbridge
GL Guei-Sheung Liu
JO Jonathan S. Oakhill
SL Shiang Y. Lim
JH Jessica K. Holien
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Identification of compound aggregation threshold was performed using DLS Zetasizer Nano ZS (Malvern Pananalytical, Worcestershire, UK). 100 µL of samples were measured in low-volume plastic micro cuvettes (BrandTech Scientific, CT, USA) at 25 °C using default size measurement settings. Buffers (PBS pH 7.4, 150 mM NaCl containing 2% or 5% DMSO with or without 0.05% Tween-20) were filtered through a 0.2 µm syringe filter prior to compound dilution. Mdivi-1 and DRP1i27 were serially diluted three-fold from 500 µM until the volume distribution of particle size was similar to that of the DMSO control. To specifically determine aggregation threshold, further dilution was carried out between concentrations where aggregation was first observed.

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