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2.6. Measurement of Nitric Oxide (NO) Content
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BV2 microglial cells were treated with amentoflavone (10 μΜ) for 1 h, followed by treatment with LPS (100 ng/ml) for 24 h. The supernatant was collected. The protein concentration was then determined with a BCA Protein Assay Kit (KeyGEN). The NO content was measured with the NO Assay Kit (Jiancheng, Nanjing, Jiangsu, China).
Copyright and License information: ©2022 Shikuo Rong et al.
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