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Aortic ring and choroidal sprouting assays.

ZL Zhiping Liu
HS Huidong Shi
JX Jiean Xu
QY Qiuhua Yang
QM Qian Ma
XM Xiaoxiao Mao
ZX Zhimin Xu
YZ Yaqi Zhou
QD Qingen Da
YC Yongfeng Cai
DF David J.R. Fulton
ZD Zheng Dong
AS Akrit Sodhi
RC Ruth B. Caldwell
YH Yuqing Huo
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Aortic ring and choroidal sprouting assays were performed as described previously (19). MRM were transfected with siCtrl or siIgf1 for 48 hours. Coculture was performed with a transwell system, with the aortic/choroidal explant cultured at the bottom of the well before adding pretreated MRM to the top of the transwells. Aortic/choroidal explant sprouting was imaged using a Zeiss LSM 780 Inverted Confocal Microscope (Carl Zeiss). The sprouting area was quantified with Adobe Photoshop.

Copyright and License information:  ©2022 Liu et al.
This work is licensed under the Creative Commons Attribution 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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