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J774.2 mouse macrophage cell line had ATTC origin. Cells were seeded in Roswell Park Memorial Institute (RPMI) medium including 10% fetal bovine serum (FBS), 1% antibiotic (100 µg/mL for each penicillin–streptomycin), and sodium pyruvate for growth. Cells were left to incubate in the incubator (5% CO2, 37 °C). The cell culture medium was changed every 4 days and made ready for the experiments [26, 27].
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