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2.6. Minimum Bactericidal Concentration (MBC) Test

EA Emad M. Abdallah
AM Abueliz Modwi
SA Samiah H. Al-Mijalli
AM Afrah E. Mohammed
HI Hajo Idriss
AO Abdulkader Shaikh Omar
MA Mohamed Afifi
AA Ammar AL-Farga
KG Khang Wen Goh
LM Long Chiau Ming
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This test was conducted to determine the lowest antibacterial agent concentration at which approximately 100% of bacterial cells are killed. MBC was obtained by sub-culturing 50 μL of each MIC tube’s test dilution on nutrient agar plates. MBC value was defined as the highest dilution that indicated no single bacterial colony. Additionally, the MBC/MIC ratio was computed [18].

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